Gobind
Khorana
Protein
Engineering
Core
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ForteBio Octet RED 96 – Bio-Layer Interferometry (BLI)
Real-time biomolecular binding analysis
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The Octet RED 96 uses Bio-Layer Interferometry (BLI) to measure binding events, kinetics, and protein concentrations in real time. Its dip-and-read workflow works with standard 96-well plates and purified or crude samples.
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Key Benefits:
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8 simultaneous channels for parallel measurements, high-throughput kinetics
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Works with crude samples: serum, lysates, viscous buffers tolerated
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Real-time kinetic measurements: Kd from ~10 pM to 1 mM; association/dissociation rates measurable
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Supports protein quantitation (e.g., IgG: 0.05–2,000 µg/mL) and small molecule binding
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Temperature controlled (15–40 °C) with orbital shaking for reproducible results
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Flexible assays: epitope binning, competition assays, and biosensor regeneration
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